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Assay of restriction endonucleases using oligonucleotides

B A Connolly1, H H Liu, D Parry

  • 1Department of Biochemistry and Genetics, Medical School, University of Newcastle upon Tyne, Newcastle upon Tyne, UK.

Methods in Molecular Biology (Clifton, N.J.)
|May 19, 2001
PubMed
Summary

No abstract available in PubMed .

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Restriction Enzymes01:11

Restriction Enzymes

Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...