Pericellular partial oxygen pressure (pO2) measurement in osteopenic bone-derived osteoblast cultures

In order to achieve further information on the in vitro behaviour of osteoblasts derived from osteopenic bone, in the present study comparative measurements of some parameters of cell proliferation, metabolism and differentiation and also of the pericellular partial oxygen pressure (pO2) were performed on normal and osteopenic bone derived osteoblasts from heathy and osteopenic rats. The respiration rate was increased in osteoblasts derived from osteopenic bone as compared to normal cells at 48 hours and 7 days, involving a significant decrease in pericellular pO2 in the culture medium. At 48 hours, in osteopenic bone-derived cells, a significant increase in MTT and a significant decrease of osteocalcin were observed. At 7 days, cell count highlighted a significant slowing down of the proliferation of osteopenic bone-derived osteoblasts. No significant differences were observed for alkaline phosphatase activity, nitric oxide and type I collagen production. The present preliminary results may be taken into consideration also in in vitro comparative biocompatibility or osteointegration studies of biomaterials in normal and osteopenic bone-derived cells because a decrease in pericellular pO2 in these tissue cultures could influence results on material behaviour.