The effect of bacterial enterotoxins implicated in SIDS on the rabbit intestine

The aim of this project was to characterise the type of damage caused to the intestine of the infant rabbit by bacterial enterotoxins implicated in sudden infant death syndrome (SIDS). Samples of the duodenum, jejunum, ileum, caecum and large intestine exposed to the toxins for up to 6 hours were examined by scanning (SEM) and transmission electron microscopy (TEM). The damage was quantitatively assessed (% villi damaged) by SEM and qualitatively by SEM and TEM. Clostridium perfringens enterotoxin, staphylococcal enterotoxin B and Clostridium difficile toxin A + toxin B combined all caused severe damage to the villi in the small intestine (80-90% damage). Clostridium difficile toxin B caused only slight damage (17% to the jejunum, 26% to the caecum). Clostridium perfringens alpha-toxin caused moderate damage to the small intestine (duodenum 34%, caecum 35%), and Escherichia coli STa caused significant damage to the small (53-70%) and large intestine (51%). The level of toxin damage increased with time, the small intestine being more susceptible generally to damage than the large intestine. Each toxin differed in its ability to damage the villi, microvilli, enterocytes and lamina propria.