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Recovery of actinorhodin from fermentation broth
1Department of Chemical Engineering, Boğaziçi University, Bebek, Istanbul, Turkey.
Journal of Chromatography. A
|May 19, 2001
Summary
A novel expanded bed chromatography method streamlines actinorhodin purification. This technique integrates multiple steps, significantly improving antibiotic recovery and processing efficiency from fermentation broth.
Area of Science:
- Biochemical Engineering
- Chromatography
- Antibiotic Purification
Background:
- Actinorhodin purification traditionally involves multiple complex steps.
- Existing methods can be time-consuming and less efficient for large-scale production.
Purpose of the Study:
- To develop a novel, single-step purification method for actinorhodin.
- To optimize expanded bed adsorption (EBA) chromatography for antibiotic recovery.
Main Methods:
- Utilized expanded bed chromatography (EBA) with various ion-exchangers (P-11, DE-52, Streamline DEAE).
- Investigated different elution strategies (isocratic vs. linear gradient) and bed expansion percentages.
- Compared breakthrough profiles between packed and expanded beds.
Main Results:
- Streamline DEAE anion-exchanger in EBA achieved 82% adsorption and 50% elution.
- Isocratic elution yielded 13.5-fold higher recovery compared to linear gradient elution.
- Expanded beds demonstrated a 33% improvement in processing volume over packed beds.
Conclusions:
- Expanded bed chromatography offers a highly efficient, integrated approach for actinorhodin purification.
- Optimized EBA parameters significantly enhance antibiotic recovery and processing capacity.
- This method presents a promising advancement for industrial-scale antibiotic production.