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Liquid chromatography-tandem mass spectrometry method of urine analysis for determining human variation in carcinogen

M G Knize¹, K S Kulp, M A Malfatti

¹Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA 94550, USA. knize1@llnl.gov

Journal of Chromatography. A

|May 19, 2001

Abstract:

We developed a solid-phase extraction LC-MS-MS method for the analysis of the four major metabolites of PhIP (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine) in human urine after a meal of well-done chicken. Ten volunteers each ate either 150 or 200 g of well-done chicken breast containing 9-21 microg of PhIP. Among the individual volunteers there is 8-fold variation in the total amount of metabolites and 20-fold variation in the relative amounts of individual metabolites, showing individual differences in carcinogen metabolism. PhIP metabolites were also detected in urine from a subject consuming chicken in a restaurant meal, demonstrating the method's sensitivity after real-life exposures.

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