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Updated: Jun 25, 2026
Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain
Published on: March 13, 2020
Reproducibility of the high-performance liquid chromatographic fingerprints obtained from two soybean cultivars and a
J Faghihi1, X Jiang, R Vierling
1Purdue University, Department of Entomology, West Lafayette, IN 47907-1158, USA.
Abstract:
Roots from soybean cultivars Williams 82 and Hartwig along with one of their progeny 14a, were extracted with non-polar, moderately polar, and highly polar solvent systems. Extracts were compared by thin-layer chromatography and by HPLC. Methanol extractions conducted at ambient temperature coupled with analysis by reversed-phase HPLC using UV detection provided the most representative sets of reproducible fingerprints. Further optimization of the overall protocol should allow for the profiling of different soybean cultivars when their roots are exposed to various environments and insults during early growth.
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