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Updated: Aug 17, 2026

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
Published on: April 8, 2016
The role of cutaneous dendritic cells in the immunopathogenesis of atopic dermatitis
C C Banfield1, R E Callard, J I Harper
1Institute of Child Health and Great Ormond Street Hospital for Children, London WC1 3JH, U.K. Cedric.Banfield@hbhc-tr.anglox.nhs.uk
Abstract:
We review the immunology of atopic dermatitis (AD) and focus attention on the role of cutaneous dendritic cells. AD is a complex immune-mediated skin disorder characterized by the recruitment of both CD4+ and CD8+ T cells into the skin. T-helper (Th) 2-type cytokines are dominant in acute AD skin, while both Th1- and Th2-type cytokines are present in chronic AD. Cutaneous dendritic cells, which are present in increased numbers within AD skin, are believed to play a key part in the activation of T cells in the skin. They may also help to determine the pattern of cytokines produced by activated effector T cells.
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