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[Angiotension I converting enzyme gene polymorphism in Chinese patients with obstructive sleep apnea syndrome]
1Sleep Disorders Center, PUMC Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100730.
Objective:
To investigate the relationship of an insertion/deletion (I/D) polymorphism of the angiotension-converting enzyme (ACE) gene to obstructive sleep apnea syndrome (OSAS).
Method:
Genomic DNA was extracted from blood samples and amplified by polymerase chain reaction (PCR). PCR primers were flanking the polymorphic region in intron 16 of the ACE gene.
Result:
The distribution of the DD, ID, and II ACE genotypes was 16%, 52%, and 32% in the control subjects and 0%, 56%, and 44% in OSAS patients, respectively. The estimated frequencies of the insertion allele and the deletion allele were 58%, 42% in the control subjects and 72%, 28% in OSAS patients, respectively. The differences were statistically significant(P < 0.05). The OSAS patients with I/I genotype had significantly longer apnea time (P < 0.05), lower minimum SaO2(P < 0.05) and more severe AHI (P < 0.05) than did the OSAS patients with I/D genotype.
Conclusion:
These results indicate that the II genotype and I allele might be a risk factor for OSAS in Chinese.