[Application of MVR-PCR at D1S8 locus with HRP-labeled MS32 oligonucleotides probes]

The purpose of this study is to detect small amount of biological materials using the method of MVR-PCR at D1S8 locus with HRP-labeled MS32 oligonucleotides probe. The results showed that the MVR-PCR patterns, obtained from trace blood stain (equal to 5 ul blood), trace saliva stain (equal to 4 ul saliva) or a hair root, can be read clearly and easily. The sensitivity of the method is 1 ng DNA. The applications of the method in personal identification were discussed.