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Updated: Aug 19, 2026

Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
[DNA content and cell cycle phase analysis in uterine sarcomas and its clinical significance]
Objective:
To investigate the relationship among DNA content, cell cycle phase analysis with the clinical-pathological features and prognosis of uterine sarcomas.
Methods:
DNA content and cell cycle phase analysis were performed on 75 paraffin-embedded specimens of uterine sarcomas using flow cytometry.
Results:
Both the aneuploidy rate and DNA index (DI) in specimens from advanced stage and poorly-differentiated cases were significantly higher than those in specimens from early stage and well-differentiated cases (P < 0.05). S-phase fraction (SPF) and proliferation index (PI) were significantly higher in advanced stage than those in early stage (P < 0.05). The 5-year suvival rates for DNA aneuploid and diploid patients were 27% and 76%, respectively (P < 0.01). The 5-year suvival rates for patients with a SPF > 20% and those with SPF < or = 20% were 10% and 56% respectively (P < 0.01).
Conclusion:
DI, SPF, PI and clinical stage are the most important factors affecting the prognosis.

