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Related Experiment Videos

Virus strain discrimination using recombinant antibodies.

N Boonham¹, I Barker

¹Central Science Laboratory, Sand Hutton, York YO41 1LZ, UK. n.boonham@csl.gov.uk

Disease Markers

|May 22, 2001

Summary

This summary is machine-generated.

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Recombinant antibody technology offers a faster, more cost-effective alternative for plant virus detection. Researchers developed single chain variable fragments (scFv) for rapid and specific identification of Potato virus Y (PVY).

Area of Science:

Plant virology
Molecular biology
Immunotechnology

Background:

Traditional monoclonal and polyclonal antibodies are standard for plant virus testing.
Conventional antibody production is time-consuming and requires expensive facilities.
Recombinant antibody technology presents a promising alternative for diagnostics.

Purpose of the Study:

To develop and evaluate single chain Fv (scFv) antibody fragments for Potato virus Y (PVY) detection.
To assess the specificity and sensitivity of selected scFv against different PVY strains.
To compare the performance of scFv in diagnostic assays with traditional monoclonal antibodies.

Main Methods:

Selection of scFv antibody fragments from a synthetic phage-antibody library.

Related Experiment Videos

Affinity selection using purified Potato virus Y, ordinary strain (PVYO).

Enzyme-Linked Immunosorbent Assay (ELISA) for virus detection in plant sap.

Main Results:

A specific scFv was selected, detecting 7 out of 9 PVYO isolates.
The selected scFv did not detect closely related PVYN and PVYNTN strains.
In ELISA, scFv detected PVY at concentrations as low as 50 ng/ml in plant sap, comparable to commercial monoclonal antibodies.

Conclusions:

Recombinant antibody technology enables rapid selection of strain-specific antibodies.
Selected scFv demonstrate high affinity and assay sensitivity for PVY diagnostics.
This technology holds significant potential for improving viral diagnostic tools in agriculture.