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[Cloning of human papillomavirus type 16 E7 gene and expression in E. coli]
Abstract
Objective:
To study the biological activity of the E7 gene of human papillomavirus 16 (HPV16).
Methods:
E7 gene from the cervical carcinoma of Chinese women was amplified by the PCR. The E7 DNA was sequenced and compared with the prototype E7 gene of HPV 16.
Results:
It showed no mutations. However when E7 gene was inserted into the prokaryotic expression vector pGEX-2T and the fused E7 protein was efficiently expressed in E. Coli (DH5 alpha). The E7 antibody could combine specifically with this fused protein by Western blot technique.
Conclusions:
The result might aid for epidemiological investigation and preparation of the vaccine.