Related Experiment Video
Updated: Oct 7, 2026
Preparation and Characterization of Novel HDL-mimicking Nanoparticles for Nerve Growth Factor Encapsulation
Published on: May 22, 2017
[Isotope tracer combined with trichloroacetic acid precipitation method for determination of nerve growth factor
Objective:
To establish the radioisotope (125I) tracer method combined with trichloroacetic acid (TCA) precipitation for the determination of nerve growth factor (NGF) in mouse plasma.
Methods:
Radioisotope tracer labelling method combined with TCA precipitation was used to determine NGF in mouse plasma.
Results:
The detection limit of the method for NGF in plasma was 2.0 ng/ml. The intra-assay cv (CV) and the inter-assay CV were all less than 10%. The assay linearity was determined over the range of 12.5-400 ng/ml in plasma and the correlation coefficient was 0.9990. The average recovery of the method for NGF was 95.21%.
Conclusions:
The radioisotope tracer method combined with TCA precipitation is stable, sensitive and reliable. It is suitable for the determination of NGF concentration in mouse plasma.
