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[Identification and study on effect of new stress protein from Neisseria gonorrhoeae]
Objective:
To investigate the influence of changing environmental growth conditions on the virulence of Neisseria gonorrhoeae.
Methods:
The gonococci were subjected to 1 mmol/L hydrogen peroxide (sublethol concentration) for one hour.
Results:
A novel gonococcal stress protein with molecular weight of 62,000 was identified by radioactive labeling and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Gonococci exposed to 1.0 mmol/L hydrogen peroxide made it significantly more resistant to higher concentrations of hydrogen peroxide and to neutrophils than control organisms (P < 0.01).
Conclusions:
These results suggest that the 62,000 stress protein might increase the virulence of N. gonorrhoeae and might be an adapted regular protein produced by N. gonorrhoeae survived within human neutrophils.