[The observation on infection of trasgenic mice (PVR Tg21) with polioviruses type 3]
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Updated: Oct 7, 2026
Viral Tracing of Genetically Defined Neural Circuitry
Published on: October 17, 2012
Objective:
To test and verify whether PVR Tg21 transgenic mice can be used as an animal model or not.
Methods:
PVR Tg21 transgenic mice were infected with 3 poliovirus strains with different neurovirulence intraspinally.
Results:
All the mice showed clinical paralysis with characteristic lesions in neurons. The symptoms varied with different potency of virus. The onset of clinical paralysis time for inoculated mice were approximately 2 days with Leon virus, 5 days with Sabin strain, and 4 days with Vaccine-321. More than 90% of mice inoculated with Leon strain died within 3 days after clinical paralysis and only 35% mice inoculated with Sabin-3 died at average of 6 days, and the PD50 are 10(1.1), 10(2.6), and 10(4.97) TCID50 and LD50 are 10(1.1), 10(3.33) and 10(5.33) TCID50, respectively. Different virus inoculated intraspinally caused different histopathological lesions.
Conclusions:
The PVR Tg21 transgenic mice could be a new animal model for neurovirulent evaluation of poliovaccines and epidemiological surveillance.
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