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Related Experiment Videos

Invasiveness and MMP expression in pancreatic carcinoma.

X Yang1, E D Staren, J M Howard

  • 1Department of Surgery, Medical College of Ohio, Toledo, Ohio 43614-5807, USA.

The Journal of Surgical Research
|May 23, 2001
PubMed
Summary

Matrix metalloproteinases (MMPs), specifically MMP-2 and MMP-9, were investigated for their role in pancreatic tumor cell invasiveness. Their expression correlated with increased invasion, suggesting a role in pancreatic cancer progression.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Matrix metalloproteinases (MMPs) are implicated in tumor invasiveness.
  • Previous research suggests a link between MMP expression and cancer progression.
  • This study specifically investigates pancreatic tumor cells and their invasiveness in relation to MMPs.

Purpose of the Study:

  • To evaluate the relationship between pancreatic tumor cell invasiveness and matrix metalloproteinases (MMPs).
  • To determine if MMP-2 and MMP-9 expression correlates with the invasive potential of pancreatic cancer cell lines.
  • To assess the effect of specific stimulants on MMP expression in pancreatic tumor cells.

Main Methods:

  • Utilized a Matrigel invasion assay with pancreatic tumor cell line SUIT-2 and its sublines.

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  • Quantified invasiveness of stimulated and unstimulated cell lines.
  • Measured matrix metalloproteinase (MMP) gene expression via RT-PCR and protein product via gelatin zymography.
  • Stimulated cells using 12-O-tetradecanoylphorbol-13-acetate (TPA), concanavalin (Con-A), and polymerized collagen type I gel (Vitrogen).

    • Main Results:

    • Pancreatic tumor cell lines exhibited varying degrees of Matrigel membrane invasion (0.4% to 6.4%).
    • Stimulants TPA, Con-A, and Vitrogen up-regulated matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) expression in specific cell lines.
    • A positive correlation was observed between Matrigel invasiveness and the up-regulation of MMP-2 and MMP-9.

    Conclusions:

    • Matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) are not constitutively expressed in pancreatic carcinoma cell lines.
    • MMP-2 and MMP-9 expression can be induced by TPA, Con-A, and Vitrogen.
    • The correlation between MMP-2 and MMP-9 expression and tumor cell invasiveness suggests their potential role in facilitating pancreatic tumor cell invasion.