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Investigation of commercial Mitolife as an antioxidant and antimutagen
J H Weisburger1, J R Hosey, E Larios
1American Health Foundation, Valhalla, New York 10595, USA. john_weisburger@nymc.edu
Abstract:
Coronary heart disease and many types of cancer are important diseases in the world and especially in Western countries. There are biochemical activation processes for low-density lipoprotein cholesterol and genotoxic carcinogens to reactive products. In part, these also involve the generation of active oxygen and reactive oxygen species. We investigated the effect of a natural product, MitoLife, which contains a mixture of fruit and tea extracts, on the oxidation of low-density lipoprotein cholesterol and the mutagenicity of five genotoxic carcinogens, specifically, 2-acetylaminofluorene, 2-aminoanthracene, 2-amino-3-methylimidazo[4,5-f]quinoline, aflatoxin B(1), and benzo[a]pyrene. A positive antioxidant control, polyphenon 60, a concentrate of green-tea polyphenols, was used to compare the effect of MitoLife with that of polyphenon. MitoLife displayed inhibiting effects in all series of tests at slightly lower effectiveness but with the same order of magnitude as the green-tea polyphenol product. Thus, MitoLife represents another means to decrease adverse effects associated with the oxidation of low-density lipoprotein cholesterol or of a series of carcinogens, some of which are in the human environment.
Insights
MitoLife, a natural fruit and tea extract, showed effectiveness in reducing low-density lipoprotein cholesterol oxidation and genotoxic carcinogen mutagenicity, similar to green tea polyphenols.
Area of Science:
- Biochemistry
- Toxicology
- Nutraceuticals
Background:
- Coronary heart disease and cancer are significant global health concerns.
- Biochemical processes involving low-density lipoprotein cholesterol and genotoxic carcinogens generate reactive oxygen species.
- Oxidative stress and mutagenicity are implicated in disease development.
Purpose of the Study:
- To evaluate the efficacy of MitoLife, a natural product containing fruit and tea extracts.
- To assess MitoLife's impact on low-density lipoprotein cholesterol oxidation.
- To determine MitoLife's effect on the mutagenicity of five specific genotoxic carcinogens.
Main Methods:
- MitoLife's antioxidant and antimutagenic properties were tested.
- Low-density lipoprotein cholesterol oxidation was measured.
- Mutagenicity assays were performed on 2-acetylaminofluorene, 2-aminoanthracene, 2-amino-3-methylimidazo[4,5-f]quinoline, aflatoxin B(1), and benzo[a]pyrene.
- Polyphenon 60 (green tea polyphenol concentrate) served as a positive antioxidant control.
Main Results:
- MitoLife demonstrated inhibitory effects in all tested oxidation and mutagenicity assays.
- The effectiveness of MitoLife was comparable in magnitude to the green tea polyphenol control.
- MitoLife exhibited slightly lower potency than the positive control but within the same order of magnitude.
Conclusions:
- MitoLife offers a potential natural intervention to mitigate risks associated with oxidized low-density lipoprotein cholesterol.
- MitoLife may help reduce adverse effects from exposure to certain environmental genotoxic carcinogens.
- This natural product represents a viable option for decreasing oxidative stress and mutagenicity.
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