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Updated: Aug 11, 2026
12:24
Murine Model of CD40-activation of B cells
Published on: March 6, 2010
Functional soluble CD100/Sema4D released from activated lymphocytes: possible role in normal and pathologic immune
X Wang1, A Kumanogoh, C Watanabe
1Department of Molecular Immunology, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
Blood
|May 23, 2001
Summary
Soluble CD100 (sCD100), a protein released by activated lymphocytes, enhances B-cell immune responses. This finding suggests sCD100 may play a role in antibody production and autoimmune diseases.
Area of Science:
- Immunology
- Molecular Biology
- Cell Biology
Background:
- CD100/Sema4D is a semaphorin family transmembrane protein crucial for immune system function.
- CD100-deficient mice exhibit impaired antibody production against T-cell-dependent antigens.
- The interaction between CD100 and CD72 is vital for immune regulation.
Purpose of the Study:
- To investigate the function of soluble CD100 (sCD100) released from activated lymphocytes.
- To determine the role of sCD100 in B-cell responses and antibody production.
- To explore the potential involvement of sCD100 in autoimmune diseases.
Main Methods:
- Detection of sCD100 in culture supernatants of activated lymphocytes.
- Affinity purification of sCD100 from activated T-cell supernatants.
- Production of recombinant sCD100-Fc fusion protein.
- Assessment of sCD100's effect on CD40-induced B-cell responses.
- Quantification of sCD100 levels in mouse sera.
Main Results:
- sCD100 was detected in activated lymphocyte cultures and sera of immunized mice.
- Recombinant and purified sCD100 significantly enhanced CD40-induced B-cell responses.
- sCD100 was present in sera of mice immunized with T-cell-dependent antigens but not T-cell-independent antigens.
- A correlation was found between sCD100 levels and autoantibody titers in MRL/lpr mice.
Conclusions:
- Soluble CD100 (sCD100) is released from activated lymphocytes and enhances B-cell immune responses.
- sCD100 may play a significant role in antibody production.
- sCD100 is implicated in the pathogenesis of autoimmune diseases.
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