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Effects of loop diuretics on angiotensin II-stimulated vascular smooth muscle cell growth
1Division of Cardiovascular Pathophysiology, School of Medicine, University of Navarra, Pamplona, Spain.
Background:
Torasemide and furosemide are diuretics that inhibit the Na(+), K(+), 2Cl(-) co-transporter localized in cells from the ascending limb of the loop of Henle. The effects of torasemide and furosemide on cell growth induced by angiotensin II (Ang II) were investigated in cultured vascular smooth muscle cells (VSMCs) obtained from the aorta of adult spontaneously hypertensive rats (SHR).
Methods:
Cell growth was determined by DNA and protein synthesis as measured by [3H]thymidine and [3H]leucine incorporation, respectively. Proliferation of VSMCs was measured using a non-radioactive colorimetric cell proliferation assay.
Results:
Ang II (10(-7) M) signficantly increased DNA and protein synthesis and cell proliferation in VSMCS: These effects were completely abolished by the Ang II type 1 receptor antagonist irbesartan (10(-6) M). Ang II-induced [3H]leucine incorporation was reduced in a dose-dependent way by torasemide (IC(50) value: 7.7+/-0.8x10(-7) M) but not by furosemide. Neither torasemide nor furosemide modified Ang II-stimulated [3H]thymidine incorporation or proliferation in VSMCs.
Conclusions:
These results indicate that torasemide, but not furosemide, inhibits Ang II-induced protein synthesis in VSMCs from SHR. Thus, it is suggested that the capacity of torasemide to block this trophic action of Ang II in rat VSMCs is not mediated by inhibition of the Na(+), K(+), 2Cl(-) co-transport mechanism.
Insights
Torasemide inhibits angiotensin II-induced protein synthesis in rat vascular smooth muscle cells, unlike furosemide. This effect is not mediated by the Na(+), K(+), 2Cl(-) co-transporter, suggesting a novel mechanism for torasemide.
Area of Science:
- Cardiovascular Research
- Pharmacology
- Nephrology
Background:
- Torasemide and furosemide are diuretics targeting the Na(+), K(+), 2Cl(-) co-transporter in the loop of Henle.
- Angiotensin II (Ang II) is implicated in vascular smooth muscle cell (VSMC) growth.
Purpose of the Study:
- To investigate the effects of torasemide and furosemide on Ang II-induced VSMC growth in spontaneously hypertensive rats (SHR).
Main Methods:
- Cultured aortic VSMCs from SHR were used.
- Cell growth assessed via [3H]thymidine and [3H]leucine incorporation (DNA and protein synthesis).
- Cell proliferation measured using a colorimetric assay.
Main Results:
- Ang II significantly increased DNA synthesis, protein synthesis, and proliferation in VSMCs.
- Torasemide dose-dependently reduced Ang II-induced protein synthesis (IC50: 7.7±0.8x10⁻⁷ M).
- Furosemide did not affect Ang II-induced protein synthesis, DNA synthesis, or proliferation.
Conclusions:
- Torasemide, but not furosemide, inhibits Ang II-induced protein synthesis in SHR VSMCs.
- This inhibitory action of torasemide is independent of the Na(+), K(+), 2Cl(-) co-transport mechanism.