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Novel pristinamycin-responsive expression systems for plant cells.

A D Frey1, M Rimann, J E Bailey

  • 1Institute of Biotechnology, Swiss Federal Institute of Technology, ETH Zurich, CH-8093 Zurich, Switzerland.

Biotechnology and Bioengineering
|May 23, 2001
PubMed
Summary

New plant gene regulation systems, PIPpOFF and PIPpON, use the antibiotic pristinamycin to control gene expression. These systems offer novel tools for plant biotechnology and research.

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Area of Science:

  • Plant Biotechnology
  • Molecular Biology
  • Synthetic Biology

Background:

  • Developing controllable gene expression systems in plants is crucial for research and biomanufacturing.
  • Existing systems often lack precise regulation or require specific inducer molecules.
  • Bacterial antibiotic resistance mechanisms can be repurposed for novel gene control strategies.

Purpose of the Study:

  • To design and characterize novel, antibiotic-inducible and -repressible gene regulation systems for plant cells.
  • To utilize the pristinamycin resistance operon components for creating synthetic plant gene switches.
  • To evaluate the performance of these systems in Nicotiana tabacum (tobacco) cell cultures.

Main Methods:

  • Constructed a pristinamycin-repressible system (PIPpOFF) using a fusion protein (PIT) and a synthetic promoter (P(pPIR)) in tobacco cells.

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  • Developed a streptogramin-inducible system (PIPpON) by combining Pip expression with a plant-specific inducible promoter (P(pPIRON)).
  • Tested both systems in Nicotiana tabacum suspension cultures to assess their functionality and responsiveness to pristinamycin.

    • Main Results:

    • The PIPpOFF system demonstrated pristinamycin-repressible activation of gene expression.
    • The PIPpON system showed successful antibiotic-induced gene expression by releasing transcriptional silencing.
    • Both systems exhibited robust performance in tobacco cell cultures, indicating their potential utility.

    Conclusions:

    • The PIPpOFF and PIPpON systems provide novel, antibiotic-controlled gene regulation in plants.
    • These systems represent a valuable addition to existing plant gene regulation technologies.
    • The developed systems hold promise for applications in basic plant science and biopharmaceutical production.