Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hsp90 levels affect telomere length in yeast.

N Grandin1, M Charbonneau

  • 1UMR CNRS 5665, Ecole Normale Supérieure, Lyon, France.

Molecular Genetics and Genomics : MGG
|May 24, 2001
PubMed
Summary

Hsc82, a heat shock protein, regulates telomere length in yeast by interacting with Cdc13 and Stn1 proteins, influencing telomerase activity and cell cycle checkpoints.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Voltage Noise Changes during Monospermic and Polyspermic Fertilization of Mature Eggs of the Anuran, Rana temporaria: (anuran egg/electrical membrane properties/fertilization/polyspermy/voltage noise).

Development, growth & differentiation·2023
Same author

Early Events in Anuran Amphibian Fertilization: An Ultrastructural Study of Changes Occurring in the Course of Monospermic Fertilization and Artificial Activation: (anuran/egg cortex/exocytosis/fertilization/microvilli).

Development, growth & differentiation·2023
Same author

The TeloDIAG: how telomeric parameters can help in glioma rapid diagnosis and liquid biopsy approaches.

Annals of oncology : official journal of the European Society for Medical Oncology·2021
Same author

Mass or total surface area with aerosol size distribution as exposure metrics for inflammatory, cytotoxic and oxidative lung responses in rats exposed to titanium dioxide nanoparticles.

Toxicology and industrial health·2016
Same author

Impact of emerging pollutants on pulmonary inflammation in asthmatic rats: ethanol vapors and agglomerated TiO2 nanoparticles.

Inhalation toxicology·2012
Same author

Alteration of infant attention and activity by polychlorinated biphenyls: unravelling critical windows of susceptibility using physiologically based pharmacokinetic modeling.

Neurotoxicology·2010

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Cdc13 protein in Saccharomyces cerevisiae binds telomeric DNA and controls telomerase.
  • Stn1 protein physically complexes with Cdc13.
  • Mutations in CDC13 and STN1 trigger DNA damage checkpoints, causing cell cycle arrest.

Purpose of the Study:

  • To identify new regulators of telomerase.
  • To investigate the role of Hsc82 in telomere maintenance.

Main Methods:

  • Two-hybrid analysis to detect protein interactions.
  • Generating and analyzing temperature-sensitive mutants.
  • Genetic screening for suppressors of stn1 mutations.
  • High-dosage screening to identify suppressors.

Main Results:

  • HSC82, encoding an Hsp90 family chaperone, was identified as a suppressor of stn1 temperature-sensitive mutations.
  • HSC82 overexpression partially rescued growth defects in cdc13-1 cells.
  • HSC82 overexpression corrected telomeric defects in stn1 mutants and shortened telomeres in wild-type cells.
  • Telomere elongation was observed in stn1-13 mutants, dependent on telomerase.

Conclusions:

  • Hsc82/Hsp82 are identified as potential regulators of telomerase activity in yeast.
  • The study highlights the involvement of molecular chaperones in telomere maintenance and regulation.

Related Experiment Videos