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Updated: Oct 7, 2026

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry
Published on: September 13, 2014
Dielectric properties of proteins from simulation: the effects of solvent, ligands, pH, and temperature
J W Pitera1, M Falta, W F van Gunsteren
1Laboratory of Physical Chemistry, Swiss Federal Institute of Technology, CH-8092 Zürich, Switzerland.
Abstract:
We have used a standard Fröhlich-Kirkwood dipole moment fluctuation model to calculate the static dielectric permittivity, epsilon(0), for four different proteins, each of which was simulated under at least two different conditions of pH, temperature, solvation, or ligand binding. For the range of proteins and conditions studied, we calculate values for epsilon(0) between 15 and 40. Our results show, in agreement with prior work, that the behavior of charged residues is the primary determinant of the effective permittivity. Furthermore, only environmental changes that alter the properties of charged residues exert a significant effect on epsilon. In contrast, buried water molecules or ligands have little or no effect on protein dielectric properties.
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