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[Study of differential expressed genes in vascular endothelial cell line ECV304 induced by low density lipoprotein]
1National Laboratory of Medical Molecular Biology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100005, China.
Objective:
To isolate and clone the differential expressed genes induced by atherogenic factors on vascular endothelium and to understand the molecular mechanism of atherosclerosis.
Methods:
The differential display reverse transcription PCR method (DDRT-PCR) was used to analyze the differential expressed cDNA in ECV304 induced by low density lipoprotein (LDL). After sequencing and homology research, the differential expressed cDNA fragments were confirmed by northern blot analysis.
Results:
Up-regulated and down-regulated cDNAs fragments were isolated in ECV304. It was found that some cDNAs fragments were highly homologous to the known human genes, while others were fragments of novel genes. The known up-regulated genes included human 10 kD protein, FGFR1 oncogene partner, intercellular adhesion molecular-1, FK506 binding protein and rTS beta genes. The known down-regulated genes included human fb19, Apobec1-binding protein 1 and RBP2. The expression levels of different genes varied by 70%-200%.
Conclusion:
By DDRT-PCR method, our results showed that LDL could change the expression of many genes in endothelial cells. The high expression level of ICAM-1 in the endothelial cells stimulated by LDL suggested that ICAM-1 is an important adhesion molecular in atherogenesis. The down-regulated expression of Apobec 1-binding protein 1 may affect the cellular synthesis of apolipoprotein. The high expression level of FGFR1 oncogene partner is likely related to the differentiation of ECV304. The function of other differential expressed genes remains to be elucidated.