Related Experiment Videos
Mouse protein Z-dependent protease inhibitor cDNA
1Division of Hematology, Barnes-Jewish Hospital at Washington University Medical Center, St Louis, MO 63110, USA.
Thrombosis and Haemostasis
|May 25, 2001
Summary
Researchers cloned mouse Protein Z-dependent protease inhibitor (ZPI) cDNA, revealing 81% amino acid homology with human ZPI and confirming its primary expression in the liver.
Area of Science:
- Biochemistry
- Molecular Biology
- Genetics
Background:
- Protein Z-dependent protease inhibitor (ZPI) is a serpin superfamily member.
- ZPI inhibits factor Xa in the presence of phospholipids, Ca++, and protein Z (PZ).
Purpose of the Study:
- To isolate and clone mouse ZPI cDNA.
- To characterize the mouse ZPI sequence and expression.
Main Methods:
- Reverse transcription-polymerase chain reaction (RT-PCR) was used to isolate mouse ZPI cDNA from liver RNA.
- Northern analysis was performed to determine mRNA size and tissue distribution.
Main Results:
- Mouse ZPI cDNA sequence analysis revealed a 1344-nucleotide open reading frame encoding a 427-amino acid mature protein.
- Mouse ZPI shares 72% nucleotide and 81% amino acid sequence homology with human ZPI.
- Mouse ZPI mRNA (1.6 kb) is predominantly expressed in the liver.
Conclusions:
- The study successfully cloned and characterized mouse ZPI cDNA.
- Mouse ZPI exhibits significant homology to human ZPI and a liver-specific expression pattern.