Effect of macrolides on the expression of HLA-DR and costimulatory molecules on antigen-presenting cells in nasal polyps
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1Department of Otolaryngology, Teikyo University School of Medicine, Tokyo, Japan.
Abstract:
To clarify the basis of macrolide therapy for improvement of chronic sinusitis, we investigated the effect of macrolides on the expression of HLA-DR and costimulatory molecules such as CD54 and CD80 on macrophages in nasal polyps. Nasal polyps taken from 54 patients who had or had not been treated with macrolides were immunohistochemically studied. The percentages of CD68-positive macrophages expressing HLA-DR or CD54 were not significantly different between patients treated with macrolides and those not treated with macrolides. However, among patients exhibiting no atopic predisposition, the number of CD80-positive macrophages was higher in patients treated with macrolides than in those not treated. In addition, the percentage of CD80-positive macrophages was negatively correlated with the percentage of infiltrating eosinophils in the polyps. These results demonstrate that macrophages act as antigen-presenting cells, expressing both major histocompatibility complex II and costimulators, and that the expression of CD80 may play a key role in the immune responses occurring in a nasal polyp. Macrolides may modulate the mucosal immune responses through CD80 expression.
Insights
Macrolide therapy may improve chronic sinusitis by affecting macrophage immune responses. Macrolides increased CD80 expression on macrophages in non-atopic patients, potentially modulating mucosal immunity.
Area of Science:
- Immunology
- Otolaryngology
- Pharmacology
Background:
- Chronic sinusitis is a common condition often treated with macrolides.
- The immunomodulatory effects of macrolides in chronic sinusitis are not fully understood.
- Macrophages play a role in the inflammatory processes of nasal polyps.
Purpose of the Study:
- To investigate the impact of macrolide therapy on macrophage-related molecules in chronic sinusitis.
- To clarify the role of HLA-DR, CD54, and CD80 expression on macrophages in nasal polyps.
- To determine how macrolides influence these molecular expressions in the context of chronic sinusitis.
Main Methods:
- Immunohistochemical analysis of nasal polyps from 54 patients.
- Comparison of macrophage marker expression (HLA-DR, CD54, CD80) between macrolide-treated and untreated groups.
- Correlation analysis between macrophage markers and eosinophil infiltration.
Main Results:
- No significant difference in HLA-DR or CD54 expression on macrophages between groups.
- Increased CD80 expression on macrophages in macrolide-treated patients without atopic predisposition.
- Negative correlation between CD80-positive macrophages and eosinophil infiltration.
Conclusions:
- Macrophages function as antigen-presenting cells in nasal polyps.
- CD80 expression on macrophages may be crucial for immune responses in nasal polyps.
- Macrolides might modulate mucosal immune responses via CD80 expression in chronic sinusitis.
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