Concordance study on population database samples using the PowerPlex 16 kit and AmpFlSTR Profiler Plus kit and...

Area of Science:

• Forensic Science
• Population Genetics
• Molecular Biology

Background:

• Forensic DNA databases rely on accurate typing of Short Tandem Repeat (STR) loci.
• Established population databases are crucial for interpreting forensic evidence.
• The reliability of typing kits across diverse populations needs continuous validation.

Purpose of the Study:

• To evaluate the reliability of PowerPlex 16 and Profiler Plus COfiler kits for forensic DNA typing.
• To establish population databases for African Americans, Bahamians, and Southwestern Hispanics at specific STR loci.
• To assess the polymorphism and utility of STR loci Penta D and Penta E.

Main Methods:

• DNA typing of over 500 samples from African Americans, Bahamians, and Southwestern Hispanics.
• Utilized PowerPlex 16 and Profiler Plus COfiler kits for genetic analysis.
• Statistical analysis using the product rule for profile rarity estimation.

Main Results:

• High concordance observed between PowerPlex 16 and Profiler Plus kits, with a single typing difference noted for FGA locus.
• Established population databases for African American, Bahamian, and Southwestern Hispanic groups.
• Penta D and Penta E STR loci demonstrated high polymorphism within these populations.

Conclusions:

• The PowerPlex 16, Profiler Plus, and COfiler kits are reliable for typing reference samples for the CODIS database.
• The newly established population databases and polymorphic STR loci (Penta D, Penta E) enhance forensic analysis capabilities.
• The product rule is applicable for estimating the rarity of complex STR profiles in these populations.