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Comparison of a radioimmunoassay with an enzymatic assay for gentamicin
Antimicrobial Agents and Chemotherapy
|January 1, 1975
Abstract:
A radioimmunoassay and an enzymatic assay for gentamicin have been compared. The correlation coefficient for results of gentamicin assays performed by the two methods with 45 serum specimens was 0.90. A similar standard curve for the radioimmunoassay was obtained with gentamicin complex, with gentamicin Cl, Cla, or C2, or with sisomicin as ligand, but tobramycin did not compete with [(3)H]gentamicin for binding to the antiserum.
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01:33Enzyme-Linked Immunosorbent Assay
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
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