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Differential cell adhesion molecule expression and lymphocyte mobilisation during prolonged aerobic exercise
G A Gannon1, S G Rhind, P N Shek
1Defence and Civil Institute of Environmental Medicine, Toronto, ON, Canada.
European Journal of Applied Physiology
|May 26, 2001
Summary
Prolonged aerobic exercise mobilizes lymphocytes, particularly CD56+, CD8hi, and CD4+ cells. This movement is linked to cell adhesion molecule CD11a expression, suggesting its role in immune response during exercise.
Area of Science:
- Immunology
- Exercise Physiology
- Cell Biology
Background:
- Immune cell dynamics during exercise are complex.
- Understanding lymphocyte mobilization is key to immune response during physical activity.
Purpose of the Study:
- To determine the cell adhesion molecule profile of CD4+, CD8hi, and CD56+ lymphocytes during prolonged aerobic exercise.
- To investigate the role of cell adhesion molecules in lymphocyte mobilization.
Main Methods:
- Two-colour flow cytometry was used to analyze lymphocyte subsets (CD4+, CD8hi, CD56+) and cell adhesion molecules (CD62L, CD49d, CD11a).
- Ten healthy males underwent prolonged aerobic exercise (2 hours at 65% peak oxygen consumption).
- Blood samples were collected at rest, during, and after exercise.
Main Results:
- Circulating concentrations of CD56+, CD8hi, and CD4+ lymphocytes significantly increased post-exercise.
- Mobilized lymphocytes were predominantly CD62L- and CD11ahi.
- Changes in lymphocyte subset concentrations correlated with CD11a surface density.
Conclusions:
- Differential lymphocyte mobilization during exercise is associated with CD11a (lymphocyte-function-associated antigen-1) expression.
- Mechanisms beyond CD11a also contribute to lymphocyte trafficking during prolonged aerobic exercise.