Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Preparing transgenic animals with a simplified method of morula aggregation using es cells.

W Kong1, P U Rani, Y Bao

  • 1The Wistar Institute, 3601 Spruce St., Philadelphia, PA 19104.

Lab Animal
|May 26, 2001
PubMed
Summary

This study introduces a new embryonic stem (ES) cell technique for producing transgenic mice, overcoming common issues with time, cost, and reliability associated with DNA microinjection methods.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

LiDAR demonstration using an InP-based optical phased array with a 3D-printed beam-shaping element.

Optics express·2026
Same author

Rubisco large and small subunits provide insights into allotetraploidy events from the genus Oryza.

Plant biology (Stuttgart, Germany)·2026
Same author

A novel BRCA mutation classification system reveals differential responses to PARP inhibition and prognostic outcomes in epithelial ovarian cancer: a multicenter study.

ESMO open·2026
Same author

CD300lf Regulates Neutrophil Aging and Periodontal Immune Homeostasis.

Journal of dental research·2025
Same author

[Policy objectives and tools for the construction of Chinese Medical Consortium: A quantitative study based on policy texts].

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences·2025
Same author

[Effective dose of remimazolam combined with oxycodone for inhibition of modified nasopharyngeal airway insertion reaction in female patients of different ages during hysteroscopic surgery].

Zhonghua yi xue za zhi·2025

Area of Science:

  • * Molecular Biology
  • * Genetics
  • * Developmental Biology

Background:

  • * Traditional methods for generating transgenic mice, such as DNA microinjection, are often time-consuming, expensive, and yield inconsistent results.
  • * These limitations hinder efficient genetic research and the development of disease models.

Purpose of the Study:

  • * To present an improved embryonic stem (ES) cell-based technique for the production of transgenic mice.
  • * To address the challenges of time, cost, and consistency inherent in current transgenic mouse generation methods.

Main Methods:

  • * Development and implementation of a novel ES cell manipulation strategy.
  • * Detailed description of the protocol for generating genetically modified ES cells.
  • * Application of the modified ES cells for creating transgenic mouse lines.

Related Experiment Videos

Main Results:

  • * The described ES cell technique significantly reduces the time required for transgenic mouse production.
  • * The method offers a more cost-effective alternative to conventional DNA microinjection.
  • * Demonstrated high consistency and reliability in generating transgenic offspring.

Conclusions:

  • * The novel ES cell technique provides a more efficient and practical approach for generating transgenic mice.
  • * This advancement facilitates broader access to genetically modified mouse models for research purposes.