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Structure of the O-specific polysaccharide of Vibrio cholerae O9 containing 2-acetamido-2-deoxy-D-galacturonic acid
N A Kocharova1, Y A Knirel, P Jansson
1Karolinska Institute, Clinical Research Centre, Huddinge University Hospital, Novum, 141 86, Huddinge, Sweden.
Carbohydrate Research
|May 30, 2001
Abstract:
The O-specific polysaccharide (OPS) was isolated by mild-acid degradation of the lipopolysaccharide of Vibrio cholerae O9 and studied by carboxyl reduction, sugar and methylation analyses, Smith degradation, and two-dimensional NMR spectroscopy, including COSY, TOCSY, NOESY, and H-detected 1H,(13)C HMQC experiments. The following structure of the pentasaccharide-repeating unit of the OPS was established: