Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene therapy by mitochondrial transfer.

Y Kagawa1, Y Inoki, H Endo

  • 1Department of Biochemistry, Jichi Medical School, Minamikawachi, Tochigi-ken, 329-0498 Japan.

Advanced Drug Delivery Reviews
|May 30, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Perioperative management of cerebral circulation].

Masui. The Japanese journal of anesthesiology·1998
Same author

Production and immunohistochemical characterization of monoclonal antibodies against ovarian antigen of the common tree shrew (Tupaia glis).

Anatomia, histologia, embryologia·1998
Same author

[Home care problems of new long-term care insurance in Japan].

Nihon Ronen Igakkai zasshi. Japanese journal of geriatrics·1998
Same author

Geographical variation of mandible size and shape in the Ryukyu wild pig (Sus scrofa riukiuanus).

The Journal of veterinary medical science·1998
Same author

Functional anatomy of gliding membrane muscles in the sugar glider (Petaurus breviceps).

Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft·1998
Same author

Sensory feedback contributes to early movement-evoked fields during voluntary finger movements in humans.

Brain research·1997

Mitochondrial DNA (mtDNA) mutations cause disease. Novel gene therapies use cytoplast fusion and microinjection to transfer healthy mtDNA, correcting defects in mutant cells.

Area of Science:

  • Genetics
  • Cell Biology
  • Molecular Medicine

Background:

  • Mitochondrial DNA (mtDNA) is prone to mutations, leading to various diseases.
  • Non-Mendelian inheritance patterns of mtDNA necessitate specialized therapeutic approaches.
  • Current gene therapy strategies for mtDNA diseases are limited.

Purpose of the Study:

  • To explore novel gene therapy techniques for mitochondrial DNA (mtDNA) diseases.
  • To present data on in vitro gene therapy using human mtDNA.
  • To evaluate the efficacy of cybrid formation and microinjection methods.

Main Methods:

  • Cytoplast fusion technique for mtDNA transfer into mutant cells.
  • Mitochondrial microinjection into oocytes for creating transmitochondrial mice.

Related Experiment Videos

  • In vitro gene therapy experiments utilizing human mtDNA.

    • Main Results:

    • Successful transfer of functional mtDNA into mutant cells via cybrid formation.
    • Demonstration of mtDNA complementation within recipient cells.
    • Data supporting the feasibility of microinjection for mitochondrial gene therapy.

    Conclusions:

    • Cytoplast fusion and mitochondrial microinjection are promising for mtDNA gene therapy.
    • These methods offer a safe and effective way to correct mtDNA defects.
    • Further research into these novel approaches is warranted for clinical applications.