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Related Experiment Video

Updated: Jul 20, 2026

Use of the Open Field Maze to Measure Locomotor and Anxiety-like Behavior in Mice
07:12

Use of the Open Field Maze to Measure Locomotor and Anxiety-like Behavior in Mice

Published on: February 6, 2015

A detailed ethological analysis of the mouse open field test: effects of diazepam, chlordiazepoxide and an extremely

E Choleris1, A W Thomas, M Kavaliers

  • 1Room 9222D, Department of Psychology, Social Science Center, University of Western Ontario, London, Ontario, Canada N6A 5C2. choleris@julian.uwo.ca

Neuroscience and Biobehavioral Reviews
|May 30, 2001
PubMed
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A detailed analysis of the open field test (OFT) reveals how drugs like diazepam and chlordiazepoxide, and magnetic fields, affect mouse anxiety behaviors. This method distinguishes specific drug effects from general activity changes.

Area of Science:

  • Behavioral Neuroscience
  • Pharmacology
  • Neuroscience

Background:

  • The open field test (OFT) is standard for assessing drug and anxiety effects.
  • Detailed ethological analysis of OFT behaviors is often lacking.
  • Understanding behavioral responses to pharmacological and non-pharmacological agents is crucial.

Purpose of the Study:

  • To conduct a detailed ethological assessment of open field behavior.
  • To investigate the effects of diazepam (DZ), chlordiazepoxide (CDP), and pulsed magnetic fields (MAG) on mouse behavior.
  • To differentiate specific anxiety-related behaviors from general activity changes.

Main Methods:

  • Male mice were treated with diazepam (1.5mg/kg) or chlordiazepoxide (5.0 and 10.0mg/kg).
  • Mice were exposed to a pulsed extremely low frequency magnetic field (MAG).

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Last Updated: Jul 20, 2026

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07:12

Use of the Open Field Maze to Measure Locomotor and Anxiety-like Behavior in Mice

Published on: February 6, 2015

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Optogenetic Manipulation of Neuronal Activity to Modulate Behavior in Freely Moving Mice

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  • Behavioral durations, frequencies, and time courses in different OFT regions were analyzed.

    • Main Results:

    • Both diazepam and chlordiazepoxide reduced anxiety-like behaviors (stretch attend, wall-following, returns) without altering total locomotion.
    • Chlordiazepoxide shifted locomotor patterns from 'high explore' to 'high walk'; diazepam affected sit and groom behaviors.
    • Magnetic field exposure reduced horizontal/vertical activity and increased grooming/spin turns, with transient reductions in anxiety behaviors.

    Conclusions:

    • Detailed ethological analysis of the OFT can identify specific effects of drugs and magnetic fields on anxiety-like behaviors.
    • This approach allows for the examination of non-specific effects on general activity.
    • The findings highlight the utility of fine-grained behavioral analysis in preclinical research.