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Updated: Aug 13, 2026

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
Published on: November 17, 2011
Lipoxygenase-dependent degradation of storage lipids
I Feussner1, H Kühn, C Wasternack
1Dept Molecular Cell Biology, Institute of Plant Genetics and Crop Plant Research, D-06466, Corrensstr. 3,., Gatersleben, Germany. feussner@ipk-gatersleben.de
Abstract:
Oilseed germination is characterized by the mobilization of storage lipids as a carbon source for the germinating seedling. In spite of the importance of lipid mobilization, its mechanism is only partially understood. Recent data suggest that a novel degradation mechanism is initiated by a 13-lipoxygenase during germination, using esterified fatty acids specifically as substrates. This 13-lipoxygenase reaction leads to a transient accumulation of ester lipid hydroperoxides in the storage lipids, and the corresponding oxygenated fatty acid moieties are preferentially removed by specific lipases. The free hydroperoxy fatty acids are subsequently reduced to their hydroxy derivatives, which might in turn undergo beta-oxidation.
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