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Related Experiment Videos

Transfection by polyethyleneimine-coated microspheres.

W S Manuel1, J I Zheng, P J Hornsby

  • 1Huffington Center on Aging and Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, TX 77030, U.S.A.

Journal of Drug Targeting
|May 30, 2001
PubMed
Summary

Polyethyleneimine (PEI)-coated microspheres show promise as a DNA delivery system. These modified microspheres effectively transfect cells, with potential for enhanced delivery through surface modifications.

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Area of Science:

  • Biomaterials Science
  • Gene Delivery
  • Nanotechnology

Background:

  • Polyethyleneimine (PEI) is a versatile cationic polymer used for DNA delivery in cell culture and in vivo.
  • Current PEI applications include surface-bound PEI and PEI/DNA microparticles for transfection.
  • Further development is needed to optimize PEI-based gene delivery systems.

Purpose of the Study:

  • To develop and evaluate PEI-coated microspheres for enhanced DNA delivery.
  • To investigate the cellular uptake and intracellular fate of PEI-coated microspheres.
  • To explore surface modifications for improved transfection efficiency.

Main Methods:

  • Preparation of microspheres using poly(epsilon-CBZ-L-lysine) and poly(D,L-lactic-co-glycolic acid) via double-emulsification/solvent evaporation.

Related Experiment Videos

  • Covalent attachment of PEI to microsphere surfaces after carbobenzoxy group removal.
  • Utilizing PEI-coated silica microspheres and assessing transfection in Caco cells and MH-S alveolar macrophages with plasmid DNA encoding green fluorescent protein.

    • Main Results:

    • PEI-coated microspheres successfully transfected Caco cells and MH-S alveolar macrophages.
    • Transfected DNA expression increased over several days post-transfection.
    • MH-S cells phagocytosed PEI-coated silica microspheres, which localized to an acidic subcellular compartment, confirmed using a pH-sensitive dye.
    • Histone complexation on microsphere surfaces enhanced MH-S cell transfection.

    Conclusions:

    • PEI-coated microspheres represent a potential DNA delivery device.
    • The unique properties of PEI combined with ease of surface modification offer advantages for gene delivery applications.