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Stem cell collection filter system for human placental/umbilical cord blood processing
M Yasutake1, M Sumita, S Terashima
1Division of Cell Processing, Institute of Medical Science, University of Tokyo, Shirokanedai 4-6-1, Minato-ku, Tokyo, 108-8639 Japan.
Vox Sanguinis
|May 31, 2001
Summary
A new stem cell collection filter system simplifies placental/umbilical cord blood processing. This method efficiently recovers nucleated cells, including valuable hematopoietic progenitor cells, for cord blood banking.
Area of Science:
- Biotechnology
- Hematology
- Cell Biology
Background:
- Established methods for placental/umbilical cord blood (PCB) volume reduction include hydroxyethyl starch and Top & Bottom methods.
- These methods are used globally for processing PCB units.
Purpose of the Study:
- To develop a simplified method for preparing nucleated cell (NC) concentrates from PCB.
- To introduce a novel filter device, the stem cell collection filter system (SCF SYSTEM), for high-recovery mononuclear cell (MNC) collection.
Main Methods:
- The SCF SYSTEM utilizes a filter and two bags, with multilayered polyethylene terephthalate non-wovens as filter media.
- PCB units were processed by gravity, with red blood cells, platelets, and plasma drained, and NCs collected via reverse washing.
- Recovered cell fractions were evaluated for viability and recovery rates.
Main Results:
- The SCF SYSTEM processed PCB units in under 30 minutes, yielding 27.4 +/- 2.2 ml of cell concentrate.
- Recovered NCs showed high viability (97.8 +/- 3.2%).
- Recovery rates for lymphocytes and monocytes were high (approx. 80%), while granulocyte recovery was lower (approx. 39%). Hematopoietic progenitor cell recovery (CD34+, colony-forming cells) was robust (approx. 80%).
Conclusions:
- The SCF SYSTEM offers an efficient and simple method for PCB processing.
- The system demonstrates good recovery of hematopoietic progenitor cells, making it suitable for cord blood banking applications.