Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Brain endothelial adhesion molecule expression in experimental colitis.

M Sans1, S Kawachi, A Soriano

  • 1Department of Gastroenterology, Hospital Clínic, IDIBAPS, Barcelona, Spain.

Microcirculation (New York, N.Y. : 1994)
|May 31, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A rare case of urinary tuberculosis in a 14-year-old: Diagnosis and surgical management.

Cirugia pediatrica : organo oficial de la Sociedad Espanola de Cirugia Pediatrica·2026
Same author

Synovial fluid Lipocalin-2 as a biomarker for chronic prosthetic joint infection: Prospective validation in hip and knee revision arthroplasty.

Revista espanola de cirugia ortopedica y traumatologia·2026
Same author

Synovial fluid Lipocalin-2 as a biomarker for chronic prosthetic joint infection: Prospective validation in hip and knee revision arthroplasty.

Revista espanola de cirugia ortopedica y traumatologia·2026
Same author

Clinical Outcomes of Intra-Abdominal Candidiasis by Initial Antifungal Therapy.

Mycoses·2026
Same author

Cefepime/enmetazobactam for Gram-negative periprosthetic joint infections? A randomized porcine study on target tissue pharmacokinetics.

The Journal of antimicrobial chemotherapy·2026
Same author

Integrative transcriptomic and functional analyses reveal candidate transcription factors associated with sorghum grain quality.

Journal of experimental botany·2026

Colitis increases brain VCAM-1 expression in animal models, but does not lead to leukocyte infiltration in the brain. This suggests specific endothelial activation without immune cell invasion during colitis.

Area of Science:

  • Neuroimmunology
  • Gastroenterology
  • Inflammation Research

Background:

  • Inflammatory bowel disease, including colitis, is associated with systemic inflammation.
  • Leukocyte adhesion molecules play a critical role in immune cell trafficking during inflammation.
  • The impact of colitis on the brain's endothelial cells and potential leukocyte infiltration remains incompletely understood.

Purpose of the Study:

  • To investigate endothelial expression of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) in the brain and other organs in four experimental colitis models.
  • To determine if leukocyte infiltration occurs in the brain of animals with experimental colitis.

Main Methods:

  • Quantification of endothelial VCAM-1 and ICAM-1 using dual radiolabeled antibody technique in rats and mice with different colitis models (TNBS, DSS, T-cell transfer, IL-10-/-).

Related Experiment Videos

  • Assessment of leukocyte infiltration in the brain via myeloperoxidase activity and immunohistochemical staining.

    • Main Results:

    • Consistent and marked upregulation of brain endothelial VCAM-1 (2- to 5.5-fold) was observed across all four colitis models.
    • Brain VCAM-1 levels strongly correlated with colon VCAM-1 and colon weight.
    • Brain ICAM-1 upregulation was limited to specific models, and no leukocyte infiltration was detected in the brain.

    Conclusions:

    • Experimental colitis induces significant and specific upregulation of endothelial VCAM-1 in the brain.
    • Cerebral endothelial cell activation in colitis models does not result in leukocyte infiltration into brain tissue.