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Myocardial gene expression of leukaemia inhibitory factor, interleukin-6 and glycoprotein 130 in end-stage human

H G Eiken1, E Øie, J K Damås

  • 1University of Oslo, The National Hospital, Ullevål Hospital Oslo, Norway. h.g.eiken@klinmed.uio.no

European Journal of Clinical Investigation
|May 31, 2001
PubMed

Insights

Interleukin-6 (IL-6) family cytokines, including leukaemia inhibitory factor (LIF), are present in human heart tissue. Elevated LIF mRNA in heart failure patients suggests its role in congestive heart failure (CHF) pathogenesis.

Area of Science:

  • Cardiovascular Biology
  • Immunology
  • Molecular Medicine

Background:

  • Interleukin-6 (IL-6) cytokine family involvement suggested in congestive heart failure (CHF) pathogenesis.
  • Previous studies utilized animal models and human plasma analyses.
  • This study investigates IL-6-related cytokines in human chronic CHF myocardium.

Purpose of the Study:

  • To examine gene and protein expression of IL-6-related cytokines in human chronic CHF.
  • To analyze myocardial tissue from end-stage heart failure patients and donor hearts.

Main Methods:

  • Gene expression analysis using cDNA array hybridization and RNase protection assays.
  • Immunohistochemistry to detect leukaemia inhibitory factor (LIF), IL-6, and glycoprotein 130 (gp130).
  • Analysis of myocardial samples from CHF patients and donor hearts.

Main Results:

  • Majority of IL-6 family cytokines and receptors showed myocardial gene activity.
  • IL-6, LIF, and gp130 localized to myocytes and vascular smooth muscle cells.
  • Significantly elevated LIF mRNA in CHF patient left ventricles compared to donors (P < 0.005).

Conclusions:

  • gp130 and its ligands IL-6 and LIF are expressed in both failing and non-failing human myocardium.
  • Elevated LIF mRNA in end-stage CHF hearts suggests a role in CHF pathogenesis.
  • Further research into LIF's role in heart failure is warranted.
Abstract

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