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TTY2: a multicopy Y-linked gene family.
1MRC Human Biochemical Genetics Unit, University College London, Wolfson House, London NW1 2HE, UK.
Genome Research
|May 31, 2001
Summary
Researchers identified the TTY2 gene family, a large group of Y-chromosome genes crucial for male fertility. These genes, some potentially noncoding RNAs or pseudogenes, are expressed in testes and kidneys.
Area of Science:
- Human genetics
- Molecular biology
- Reproductive biology
Background:
- The Y chromosome plays a critical role in male sex determination and sperm production.
- Identifying Y-linked genes expressed in the testis is essential for understanding male fertility.
Purpose of the Study:
- To identify Y-linked, testis-expressed genes.
- To characterize the TTY2 gene family and its organization on the Y chromosome.
Main Methods:
- cDNA selection using Y-cosmid clones.
- Sequencing of selected cDNA clones.
- Screening of Y-linked cosmids.
- RT-PCR analysis.
Main Results:
- A cDNA library identified 19 clones homologous to TTY2, indicating a large gene family.
- The TTY2 gene family comprises at least 26 members in 14 subfamilies.
- TTY2 genes are organized in tandem clusters on both Y chromosome arms.
- Two TTY2 genes were expressed in adult/fetal testis and adult kidney.
- Investigated genes lack open reading frames.
Conclusions:
- The TTY2 gene family is a multigene family on the Y chromosome.
- Some TTY2 family members may function as noncoding RNAs.
- Other TTY2 family members may be pseudogenes.
- The expression in testis and kidney suggests potential roles beyond spermatogenesis.