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Circulating skeletal stem cells.

S A Kuznetsov1, M H Mankani, S Gronthos

  • 1Craniofacial and Skeletal Diseases Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, 30 Convent Drive, Bethesda, MD 20892, USA. skuznets@mail.nih.gov

The Journal of Cell Biology
|May 31, 2001
PubMed
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Researchers discovered circulating skeletal stem cells in mammalian blood. These blood-borne cells possess osteogenic and adipogenic potential, differentiating into bone cells after transplantation.

Area of Science:

  • Stem cell biology
  • Hematology
  • Regenerative medicine

Background:

  • Skeletal stem cells are crucial for bone formation and repair.
  • Traditionally, skeletal stem cells were primarily isolated from bone marrow (stromal stem cells or mesenchymal stem cells).
  • The presence and function of circulating skeletal stem cells in mammals remained unconfirmed.

Purpose of the Study:

  • To isolate and characterize fibroblast-like cells from mammalian blood.
  • To investigate the osteogenic and adipogenic potential of these blood-derived cells.
  • To provide definitive proof of circulating skeletal stem cells in mammals.

Main Methods:

  • Isolation of adherent, clonogenic, fibroblast-like cells from the blood of four mammalian species.
  • In vivo transplantation assay using immunocompromised mice.

Related Experiment Videos

  • Species-specific probes for donor origin confirmation of differentiated bone cells.

    • Main Results:

    • Successfully isolated blood-borne cells with fibroblast-like morphology and clonogenicity.
    • Demonstrated osteogenic and adipogenic potential of these cells in vitro.
    • Confirmed in vivo osteogenic potential through successful bone formation after transplantation.
    • Verified donor origin of newly formed bone cells using species-specific probes.

    Conclusions:

    • This study provides the first definitive evidence for the existence of circulating skeletal stem cells in mammals.
    • These blood-derived cells share characteristics with bone marrow stromal stem cells but are distinguishable.
    • The findings open new avenues for regenerative medicine and understanding skeletal development and repair.