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Updated: Jul 14, 2026
11:27
Studying DNA Looping by Single-Molecule FRET
Published on: June 28, 2014
Fidelity of enzymatic ligation for DNA computing
D Faulhammer1, R J Lipton, L F Landweber
1Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ 08544, USA.
Abstract:
We describe a convenient assay for rapid qualitative evaluation of hybridization/ligation fidelity. The approach uses randomized probe strands of DNA and restriction enzyme digestion after amplification of reaction products by the polymerase chain reaction (PCR). We report ligation efficiencies and fidelities of two DNA ligases, T4 DNA ligase and Thermus aquaticus (Taq) DNA ligase, over a range of temperatures.
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