Related Experiment Video

Updated: Jun 29, 2026

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
11:24

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

Published on: May 13, 2017

Laser raman spectroscopy--new probe of myosin substructure

E B Carew, I M Asher, H E Stanley

    Science (New York, N.Y.)
    |May 30, 1975
    PubMed

    More Related Videos

    Raman and IR Spectroelectrochemical Methods as Tools to Analyze Conjugated Organic Compounds09:11

    Raman and IR Spectroelectrochemical Methods as Tools to Analyze Conjugated Organic Compounds

    Published on: October 12, 2018

    Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features07:09

    Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features

    Published on: March 16, 2022

    Related Concept Videos

    IR and UV–Vis Spectroscopy of Carboxylic Acids01:28

    IR and UV–Vis Spectroscopy of Carboxylic Acids

    In IR spectroscopy of carboxylic acids, the C=O bond shows a characteristic band between 1710 and 1760 cm⁻¹, and the O–H bond exhibits a broad band between 2500 and 3300 cm⁻¹.
    However, the stretching absorptions for the C=O bond vary depending on the structure of carboxylic acids. The C=O bond of the free carboxylic acids shows a higher stretching frequency, 1760 cm−1, while H-bonded carboxylic acids (dimers) exhibit stretching absorptions at a lower frequency, 1710 cm−1. The C=O bond of the...
    Spectroscopy of Carboxylic Acid Derivatives01:26

    Spectroscopy of Carboxylic Acid Derivatives

    Infrared spectroscopy is primarily used to determine the types of bonds and functional groups. In carboxylic acid derivatives, a typical carbonyl bond absorption is observed around 1650–1850 cm−1. For esters, the absorption is recorded at around 1740 cm−1, while acid halides show the absorption at about 1800 cm−1. Another acid derivative, the acid anhydrides, exhibit two carbonyl absorption around 1760 cm−1 and 1820 cm−1, arising from the symmetrical and unsymmetrical carbonyl vibration.
    In the...
    Mass Spectrometry of Amines01:15

    Mass Spectrometry of Amines

    In mass spectroscopy, amines undergo fragmentation to give parent ions with odd molecule weights. This observed mass spectrum follows the nitrogen rule; a molecule with an odd number of nitrogen atoms produces a molecular ion with an odd molecular weight. Amines undergo fragmentation through α cleavage, producing nitrogen-containing cations—iminium ions—and alkyl radicals. Mass spectra of aromatic and cyclic aliphatic amines exhibit strong molecular ion peaks, but acyclic aliphatic amines show...
    High-Performance Liquid Chromatography: Types of Detectors01:15

    High-Performance Liquid Chromatography: Types of Detectors

    The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
    Raman Spectroscopy Instrumentation: Overview01:26

    Raman Spectroscopy Instrumentation: Overview

    A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
    The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...