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Related Experiment Videos

Cell surface events during resealing visualized by scanning-electron microscopy.

P L McNeil1, M M Baker

  • 1Department of Cellular Biology and Anatomy, and Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta 30912, USA. pmcneil@mail.mcg.edu

Cell and Tissue Research
|June 1, 2001
PubMed
Summary

Exocytosis aids plasma membrane resealing by patching disruptions with internal membranes, not by lipid flow. This patching mechanism is crucial for repairing large membrane tears in sea urchin eggs.

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Area of Science:

  • Cell Biology
  • Membrane Dynamics
  • Wound Healing

Background:

  • Exocytosis is hypothesized to play a role in plasma membrane resealing.
  • Two potential mechanisms include lipid flow or membrane patching.

Purpose of the Study:

  • To investigate the role of exocytosis in plasma membrane resealing.
  • To differentiate between lipid flow and membrane patching mechanisms.

Main Methods:

  • Inducing plasma membrane disruptions via shear stress in sea urchin eggs.
  • Utilizing scanning electron microscopy to visualize disruption sites.
  • Manipulating external calcium (Ca2+) levels to control resealing.

Main Results:

  • In the absence of Ca2+ (preventing resealing), disruptions were filled with uniform small vesicles.

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  • In the presence of Ca2+ (allowing resealing), disruptions showed heterogeneous, enlarged vesicles, some >10 micrometers.
  • Evidence for lipid flow (single apertures) was absent; instead, large vesicles fused and integrated with the plasma membrane.

    • Conclusions:

    • The "patching" mechanism, involving the fusion of internal membrane vesicles, is the primary driver of plasma membrane resealing.
    • Exocytosis contributes to resealing by providing these internal membrane patches to repair large disruptions.