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Detecting the Lyme Disease Spirochete, Borrelia Burgdorferi, in Ticks Using Nested PCR
Published on: February 4, 2018
Risk for human tick-borne encephalitis, borrelioses, and double infection in the pre-Ural region of Russia
E I Korenberg1, L Y Gorban, Y V Kovalevskii
1Gamaleya Research Institute for Epidemiology and Microbiology, Russian Academy of Medical Sciences, Moscow, Russia.
Abstract:
We assessed the risk for human tick-borne encephalitis (TBE), ixodid tick-borne borrelioses, and double infection from 1994 to 1998 in Perm, which has among the highest rates of reported cases in Russia. We studied 3,473 unfed adult Ixodes persulcatus ticks collected from vegetation in natural foci and 62,816 ticks removed from humans. TBE virus and Borrelia may coexist in ticks.
Insights
This study investigated tick-borne encephalitis (TBE) and borrelioses risk in Perm, Russia. TBE virus and Borrelia bacteria were found to coexist in Ixodes persulcatus ticks, indicating potential for co-infection.
Area of Science:
- * Veterinary Entomology
- * Infectious Diseases Epidemiology
- * Medical Zoology
Background:
- * Perm, Russia, exhibits high incidence rates of tick-borne diseases.
- * Ixodid ticks are vectors for serious human pathogens, including TBE virus and Borrelia species.
- * Understanding co-infection risks is crucial for public health management.
Purpose of the Study:
- * To assess the risk of tick-borne encephalitis (TBE), borrelioses, and co-infections in Perm, Russia.
- * To investigate the presence and potential coexistence of TBE virus and Borrelia in Ixodes persulcatus ticks.
Main Methods:
- * Analysis of 3,473 unfed adult Ixodes persulcatus ticks collected from natural foci.
- * Examination of 62,816 ticks removed from human hosts between 1994 and 1998.
- * Detection of TBE virus and Borrelia spp. in tick populations.
Main Results:
- * Ixodes persulcatus ticks collected in Perm were found to harbor TBE virus and Borrelia.
- * Evidence suggests the potential for simultaneous presence of TBE virus and Borrelia within individual ticks.
- * The study period (1994-1998) highlights ongoing public health concerns in the region.
Conclusions:
- * Co-circulation of TBE virus and Borrelia in tick populations poses a significant risk for human co-infection.
- * Ixodes persulcatus ticks in Perm are confirmed vectors for both TBE and borrelioses.
- * Public health strategies should consider the dual threat of these tick-borne pathogens.
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