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Rescue of mouse embryos from 2-cell blocks by microinjection of maturation promoting factor
1First Department of Obstetrics and Gynecology, Toho University School of Medicine, Tokyo, Japan. hide-n@jg7.so-net.ne.jp
Objective:
To examine the rescue of mouse embryos from 2-cell blocks by the microinjection of maturation promoting factor (MPF) extracted from matured Xenopus eggs into one of the blastomeres of 2-cell stage mouse embryos.
Design:
Controlled laboratory study.
Setting:
First Department of Obstetrics and Gynecology, Toho University School of Medicine, Tokyo, Japan.
Animal(S):
Eight- to 10-week-old female Crj:CD-1(ICR) mice.
Intervention(S):
One of the blastomeres of the mouse 2-cell embryos was injected with MPF (MI group) or mHTF medium (MED group) at 28--32 hours after insemination.
Main Outcome Measure(S):
The developmental rate to blastocyst.
Result(S):
The developmental rate to blastocyst in the MI group (48.0%) was significantly higher than that in the MED group (0%).
Conclusion(S):
The 2-cell block was specifically rescued by the microinjection of MPF and not by the insertion of pipettes.