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A technique for laparoscopic transplantation of frozen-banked ovarian tissue
K Oktay1, B A Aydin, G Karlikaya
1Center For Reproductive Medicine and Infertility, Weill Medical College of Cornell University, New York, New York 10021, USA.kuo9001@med.cornell.edu
Objective:
To describe a laparoscopic technique for transplanting frozen-banked ovarian tissue.
Design:
Case study.
Setting:
Academic medical center.
Patient(S):
A patient whose ovarian tissue was previously frozen with the slow-freeze technique.
Intervention(S):
Microsurgical reconstruction of ovarian cortex and its laparoscopic transplantation to the ovarian fossa.
Main Outcome Measure(S):
Blood flow to the grafts by doppler, follicle development and ovulation by ultrasound, E(2) and progesterone production, and resumption of spontaneous menses.
Result(S):
The patient ovulated and menstruated 4 months after the transplant in response to ovarian stimulation with menopausal gonadotropins.
Conclusion(S):
Laparoscopic transplantation of frozen-banked ovarian tissue beneath the pelvic peritoneum can restore ovarian function.