A new angiotensinergic system in the CNS of the rat

The use of two different polyclonal, affinity-purified, monospecific antibodies to ANG II (called BODE and BODE 1) revealed dissimilar distribution of ANG II immunoreactivity within the rat central nervous system (CNS). The ANG II-like material detected using BODE was concentrated in the neurosecretory hypothalamic nuclei, in the inner layer of the median eminence and in the posterior lobe of the pituitary. In contrast, the BODE 1 antibody did not stain the hypothalamic-neurohypophysial angiotensinergic system, and the staining pattern was much more broadly distributed throughout the CNS. BODE 1 is the first antibody that can be used to verify the locations of endogenous angiotensin and their receptor sites in the CNS. The diverse distribution of the ANG II-like material detected by the two antibodies provides strong evidence for the existence of at least two different angiotensinergic systems in the CNS.