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Related Experiment Videos

Human telomerase reverse transcriptase expression in Diff-Quik-stained FNA samples from thyroid nodules.

M T Siddiqui1, K L Greene, D P Clark

  • 1Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B
|June 1, 2001
PubMed
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Detecting human telomerase reverse transcriptase (hTERT) gene expression in archival thyroid fine-needle aspiration (FNA) slides is feasible. This method aids in differentiating benign from malignant thyroid nodules, especially in indeterminate cases.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pathology

Background:

  • Fine-needle aspiration (FNA) is crucial for diagnosing thyroid nodules, but 10% yield indeterminate results.
  • Human telomerase reverse transcriptase (hTERT) gene expression has shown promise in distinguishing benign from malignant thyroid lesions.
  • The feasibility of detecting hTERT gene expression in archival FNA specimens requires investigation.

Purpose of the Study:

  • To determine if hTERT gene expression can be detected in archival Diff-Quik-stained thyroid FNA specimens using reverse transcription-polymerase chain reaction (RT-PCR).
  • To assess the utility of hTERT gene expression detection in improving the diagnostic accuracy of indeterminate thyroid FNAs.

Main Methods:

  • RNA extraction from archival Diff-Quik-stained thyroid FNA samples.

Related Experiment Videos

  • RT-PCR analysis for hTERT gene expression.
  • Comparison of RT-PCR results with cytopathologic diagnoses and frozen sample analyses.
  • Evaluation of the impact of specimen age on RT-PCR success.

    • Main Results:

    • Successful RT-PCR for hTERT was achieved in 48 of 58 (83%) archival FNA specimens.
    • Specimens older than 3 years showed a lower success rate for RT-PCR.
    • hTERT expression varied across different thyroid nodule types, with higher detection rates in malignant lesions.
    • RT-PCR results for hTERT generally correlated with those from corresponding frozen samples.

    Conclusions:

    • Diff-Quik-stained thyroid FNA specimens under 3 years old are suitable for hTERT gene expression detection via RT-PCR.
    • Detecting hTERT gene expression can enhance the diagnostic capability for indeterminate thyroid FNA samples.
    • This molecular marker, combined with cytopathology, may improve the differentiation of benign and malignant thyroid nodules.