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Rice genomics: current status of genome sequencing
1Rice Genome Research Program, National Institute of Agrobiological Resources, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
Novartis Foundation Symposium
|June 5, 2001
Summary
The Rice Genome Research Program developed essential tools for rice genome analysis and launched a sequencing project. They created a physical map and published 1.9 Mb of sequenced rice DNA, advancing rice genomics.
Area of Science:
- Genomics
- Molecular Biology
- Plant Science
Background:
- The Rice Genome Research Program (RGP) was established in 1991.
- RGP has developed foundational tools for rice genome analysis, including cDNA catalogs and physical maps.
- An international rice genome sequencing project was initiated by RGP in 1998.
Purpose of the Study:
- To advance rice genomics through large-scale genome sequencing.
- To construct a sequence-ready physical map for efficient genome analysis.
- To publish assembled genomic sequences for public access and further research.
Main Methods:
- Construction of a P1-derived artificial chromosome (PAC)-based physical map.
- Utilizing expressed sequence tag (EST) markers derived from cDNA sequences.
- Shotgun sequencing of selected PAC clones with 100-150 kb inserts from chromosomes 1 and 6.
- Gene prediction on assembled genomic sequences.
Main Results:
- A PAC-based, sequence-ready physical map was successfully constructed.
- Shotgun sequencing was performed on selected PAC clones.
- Assembled genomic sequences, including predicted gene-coding regions, were published.
- As of January 2000, 1.9 Mb of genomic sequence from 13 PAC clones were publicly available.
Conclusions:
- The RGP has made significant progress in rice genome sequencing and analysis.
- Published genomic data provides a valuable resource for the scientific community.
- Future prospects focus on understanding rice genomic information at the nucleotide level.