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Novel method using phytase for separating soybean beta-conglycinin and glycinin
1New Ingredients Research Institute, Tsukuba R&D Center, Fuji Oil Co. Ltd., Tsukuba-gun, Ibaraki, Japan. saituto@fujioil.co.jp
Bioscience, Biotechnology, and Biochemistry
|June 5, 2001
Abstract:
A novel method for separating soybean beta-conglycinin and glycinin from defatted soymilk by a phytase treatment was developed. Phytase was added to defatted soymilk (1000FYT/100 g of protein) at pH 6.0, and the mixture incubated for 1 h at 40 degrees C. This procedure separated beta-conglycinin and glycinin without needing a reducing agent or cooling into the soluble and insoluble fractions, respectively. Simultaneously, most of the phytate in both proteins was removed.