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Auxin distribution and transport during embryonic pattern formation in wheat
C Fischer-Iglesias1, B Sundberg, G Neuhaus
1University of Freiburg, Institute of Biology II, Cell Biology, Schänzlestrasse 1, D-79104 Freiburg, Germany. Fischer_Iglesis@hotmail.com
The Plant Journal : for Cell and Molecular Biology
|June 8, 2001
Summary
Plant embryo development relies on auxin distribution. This study shows auxin transport pathways change from radial to bilateral symmetry, influencing embryonic pattern formation in wheat.
Area of Science:
- Plant Biology
- Developmental Biology
- Molecular Biology
Background:
- Auxin's role in plant embryogenesis is crucial for pattern formation.
- Specific spatial auxin distribution is vital for establishing embryonic symmetry.
Purpose of the Study:
- To visualize auxin distribution in wheat embryos using a photoaffinity labeling agent.
- To deduce auxin transport pathways and their role in embryonic development.
Main Methods:
- Utilized tritiated 5-azidoindole-3-acetic acid ([3H],5-N3IAA), an indole-3-acetic acid (IAA) analog.
- Visualized auxin distribution in in vitro and in planta grown wheat embryos.
- Applied N-1-naphthylphthalamic acid, an auxin polar transport inhibitor.
Main Results:
- Demonstrated heterogeneous auxin distribution that changes during wheat embryo development.
- Correlated the shift from radial to bilateral symmetry with auxin redistribution.
- Showed auxin polar transport inhibition affects auxin localization in bilaterally symmetrical embryos.
Conclusions:
- The transition to bilateral symmetry is linked to the initiation of directed auxin transport.
- Auxin redistribution is a key factor in establishing embryonic pattern.
- IAA concentration increases during development, suggesting synthesis or release in later stages.